A thermostable archaeal S-adenosylmethionine synthetase: a promising tool to improve the synthesis of adenosylmethionine analogs of biotechnological interest

Bioengineered. 2015;6(3):184-6. doi: 10.1080/21655979.2015.1045170.

Abstract

The naturally and widely occurring sulfonium compound, S-adenosylmethionine (AdoMet), one of nature's most versatile molecules, is biosynthesized from methionine and ATP by AdoMet synthetase or methionine adenosyltransferase (MAT) in a 2-step reaction in which the energy-rich sulfonium compound is formed by dephosphorylation of ATP. All living cells, with the only exception of some parasites and infectious agents, express MAT.

Keywords: AdoMet, S-Adenosylmethionine; Archaea; BsMAT, MAT from Bacillus subtilis; DMSO, dimethylsulfoxide; MAT, adenosylmethionine synthetase or methionine adenosyltransferase; EcMAT, MAT from E. coli.; MjMAT, MAT from Methanococcus jannaschii; PfMAT, MAT from Pyrococcus furiosus; Pyrococcus furiosus; S-Adenosylmethionine; SsMAT, MAT from Sulfolobus solfataricus; TkMAT, MAT from Thermococcus kodakarensis; adenosylmethionine synthetase; hyperthermophilic enzymes; methionine adenosyltransferase; organic solvent activity and thermostability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / metabolism
  • ATP Synthetase Complexes / metabolism
  • Archaea / enzymology*
  • Chemical Phenomena
  • Industrial Microbiology*
  • Methionine Adenosyltransferase / metabolism*
  • S-Adenosylmethionine / analogs & derivatives*
  • S-Adenosylmethionine / biosynthesis*

Substances

  • S-Adenosylmethionine
  • 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
  • Methionine Adenosyltransferase
  • ATP Synthetase Complexes