Fat and carbohydrate in western diet contribute differently to hepatic lipid accumulation

Biochem Biophys Res Commun. 2015 Jun 12;461(4):681-6. doi: 10.1016/j.bbrc.2015.04.092. Epub 2015 Apr 27.

Abstract

We investigated the contributions of dietary fat and dietary carbohydrate to the development of fatty liver induced by western diet (WD). Compared with WD-fed wild type (WT) mice, livers of WD-fed ChREBP(-/-) mice showed lipid droplets of varying sizes around the hepatic lobules, while hepatic triglyceride and cholesterol contents were only modestly decreased. Inflammation and fibrosis were suppressed in ChREBP(-/-) mice. In addition, compared with WD-fed WT mice, ChREBP(-/-) mice showed decreased β-oxidation, ketogenesis and FGF21 production, increased intestinal lipid absorption, and decreased VLDL secretion. These findings suggest that dietary fat and carbohydrate contribute differently to the development of fatty liver.

Keywords: ChREBP; FGF21; Hepatic steatosis; Ketogenesis; VLDL; β-oxidation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Diet, Western*
  • Dietary Carbohydrates / metabolism*
  • Dietary Fats / metabolism*
  • Fatty Liver / metabolism*
  • Lipid Metabolism / physiology*
  • Liver / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Dietary Carbohydrates
  • Dietary Fats
  • Mlxipl protein, mouse
  • Nuclear Proteins
  • Transcription Factors