Opposite expression of CYP51A1 and its natural antisense transcript AluCYP51A1 in adenovirus type 37 infected retinal pigmented epithelial cells

Julia Maria Anna Pickl; Wael Kamel; Sibel Ciftci; Tanel Punga; Göran Akusjärvi

doi:10.1016/j.febslet.2015.04.018

Opposite expression of CYP51A1 and its natural antisense transcript AluCYP51A1 in adenovirus type 37 infected retinal pigmented epithelial cells

FEBS Lett. 2015 May 22;589(12):1383-8. doi: 10.1016/j.febslet.2015.04.018. Epub 2015 Apr 20.

Authors

Julia Maria Anna Pickl¹, Wael Kamel¹, Sibel Ciftci¹, Tanel Punga¹, Göran Akusjärvi²

Affiliations

¹ Department of Medical Biochemistry and Microbiology, Uppsala University, Husargatan 3, BMC, Box 582, 75123 Uppsala, Sweden.
² Department of Medical Biochemistry and Microbiology, Uppsala University, Husargatan 3, BMC, Box 582, 75123 Uppsala, Sweden. Electronic address: goran.akusjarvi@imbim.uu.se.

PMID: 25907535
DOI: 10.1016/j.febslet.2015.04.018

Abstract

Cytochrome P450 family member CYP51A1 is a key enzyme in cholesterol biosynthesis whose deregulation is implicated in numerous diseases, including retinal degeneration. Here we describe that HAdV-37 infection leads to downregulation of CYP51A1 expression and overexpression of its antisense non-coding Alu element (AluCYP51A1) in retinal pigment epithelium (RPE) cells. This change in gene expression is associated with a reversed accumulation of a positive histone mark at the CYP51A1 and AluCYP51A1 promoters. Further, transient AluCYP51A1 RNA overexpression correlates with reduced CYP51A1 mRNA accumulation. Collectively, our data suggest that AluCYP51A1 might control CYP51A1 gene expression in HAdV-37-infected RPE cells.

Keywords: Adenovirus; Alu element; CYP51A1; Human adenovirus type 37; Retinal pigment epithelium.

Publication types

Research Support, Non-U.S. Gov't
Comment

MeSH terms

Mechanical Phenomena*
Microscopy, Atomic Force / methods*
Molecular Imaging / methods*