Changes of TIZ expression in epithelial ovarian cancer cells

Asian Pac J Trop Med. 2015 Feb;8(2):157-61. doi: 10.1016/S1995-7645(14)60308-4.

Abstract

Objective: To study the change of TIZ expression in epithelial ovarian cancer cells.

Methods: HO8910 cells were transinfected with siRNA to inhibit the expression of TIZ. pcDNA3.1-TIZ vectors were combined to increase the TIZ expression level. The cell viability, colony forming efficiency and cycle distribution of HO8910, HO8910/NC, HO8910/pcDNA3.1-NC, HO8910/TIZ-573 and H08910/pcDNA3.1-TIZ were compared, and the invasion rate, migration rate and adhesion rate between 5 groups of cells were compared.

Results: Compared with those of HO8910, HO8910/NC and HO8910/pcDNA3.1-NC, the cell viability, colony forming efficiency and cell cycle distribution of HO8910/TIZ-573 were increased, while the indexes of H08910/pcDNA3.1-NC were decreased with statistical significant difference (P<0.05). There was no statistical significant difference in the invasion rate, migration rate and adhesion rate between 5 groups of cells (P>0.05).

Conclusions: The expression of TIZ can inhibit the proliferation of epithelial ovarian cancer cells.

Keywords: Cell proliferation; Epithelial ovarian cancer cells; Invasion ability; TIZ expression.