Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells

Miina Ojansivu; Sari Vanhatupa; Leena Björkvik; Heikki Häkkänen; Minna Kellomäki; Reija Autio; Janne A Ihalainen; Leena Hupa; Susanna Miettinen

doi:10.1016/j.actbio.2015.04.017

Bioactive glass ions as strong enhancers of osteogenic differentiation in human adipose stem cells

Acta Biomater. 2015 Jul:21:190-203. doi: 10.1016/j.actbio.2015.04.017. Epub 2015 Apr 18.

Authors

Miina Ojansivu¹, Sari Vanhatupa², Leena Björkvik³, Heikki Häkkänen⁴, Minna Kellomäki⁵, Reija Autio⁶, Janne A Ihalainen⁴, Leena Hupa³, Susanna Miettinen⁷

Affiliations

¹ Adult Stem Cell Research Group, University of Tampere, Tampere, Finland; BioMediTech, University of Tampere and Tampere University of Technology, Tampere, Finland; Science Centre, Tampere University Hospital, Tampere, Finland. Electronic address: miina.ojansivu@uta.fi.
² Adult Stem Cell Research Group, University of Tampere, Tampere, Finland; BioMediTech, University of Tampere and Tampere University of Technology, Tampere, Finland; Science Centre, Tampere University Hospital, Tampere, Finland.
³ Johan Gadolin Process Chemistry Centre, Åbo Akademi University, Turku, Finland.
⁴ Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland.
⁵ BioMediTech, University of Tampere and Tampere University of Technology, Tampere, Finland; Biomaterials and Tissue Engineering Group, Department of Electronics and Communications Engineering, Tampere University of Technology, Tampere, Finland.
⁶ School of Health Sciences, University of Tampere, Tampere, Finland.
⁷ Adult Stem Cell Research Group, University of Tampere, Tampere, Finland; BioMediTech, University of Tampere and Tampere University of Technology, Tampere, Finland; Science Centre, Tampere University Hospital, Tampere, Finland; Science Centre, Tampere University Hospital, Tampere, Finland.

PMID: 25900445
DOI: 10.1016/j.actbio.2015.04.017

Abstract

Bioactive glasses are known for their ability to induce osteogenic differentiation of stem cells. To elucidate the mechanism of the osteoinductivity in more detail, we studied whether ionic extracts prepared from a commercial glass S53P4 and from three experimental glasses (2-06, 1-06 and 3-06) are alone sufficient to induce osteogenic differentiation of human adipose stem cells. Cells were cultured using basic medium or osteogenic medium as extract basis. Our results indicate that cells stay viable in all the glass extracts for the whole culturing period, 14 days. At 14 days the mineralization in osteogenic medium extracts was excessive compared to the control. Parallel to the increased mineralization we observed a decrease in the cell amount. Raman and Laser Induced Breakdown Spectroscopy analyses confirmed that the mineral consisted of calcium phosphates. Consistently, the osteogenic medium extracts also increased osteocalcin production and collagen Type-I accumulation in the extracellular matrix at 13 days. Of the four osteogenic medium extracts, 2-06 and 3-06 induced the best responses of osteogenesis. However, regardless of the enhanced mineral formation, alkaline phosphatase activity was not promoted by the extracts. The osteogenic medium extracts could potentially provide a fast and effective way to differentiate human adipose stem cells in vitro.

Keywords: Bioactive glass; Bone tissue engineering; Mesenchymal stem cell; Mineralization; Osteogenic differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue / cytology*
Biocompatible Materials*
Cell Differentiation*
Cell Proliferation
Glass*
Humans
Ions
Stem Cells / cytology*

Substances

Biocompatible Materials
Ions