Isolation of a sesquiterpene synthase expressing in specialized epithelial cells surrounding the secretory cavities in rough lemon (Citrus jambhiri)

Yuya Uji; Rika Ozawa; Hodaka Shishido; Shiduku Taniguchi; Junji Takabayashi; Kazuya Akimitsu; Kenji Gomi

doi:10.1016/j.jplph.2015.03.013

Isolation of a sesquiterpene synthase expressing in specialized epithelial cells surrounding the secretory cavities in rough lemon (Citrus jambhiri)

J Plant Physiol. 2015 May 15:180:67-71. doi: 10.1016/j.jplph.2015.03.013. Epub 2015 Apr 3.

Authors

Yuya Uji¹, Rika Ozawa², Hodaka Shishido¹, Shiduku Taniguchi¹, Junji Takabayashi², Kazuya Akimitsu¹, Kenji Gomi³

Affiliations

¹ Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan.
² Center for Ecological Research, Kyoto University, Otsu, Shiga 520-2113, Japan.
³ Faculty of Agriculture and Gene Research Center, Kagawa University, Miki, Kagawa 761-0795, Japan. Electronic address: gomiken@ag.kagawa-u.ac.jp.

PMID: 25899729
DOI: 10.1016/j.jplph.2015.03.013

Abstract

Volatile terpenoids such as monoterpenes and sesquiterpenes play multiple roles in plant responses and are synthesized by terpene synthases (TPSs). We have previously isolated a partial TPS gene, RlemTPS4, that responds to microbial attack in rough lemon. In this study, we isolated a full length RlemTPS4 cDNA from rough lemon. RlemTPS4 localized in the cytosol. The recombinant RlemTPS4 protein was obtained using a prokaryotic expression system and GC-MS analysis of the terpenes produced by the RlemTPS4 enzymatic reaction determined that RlemTPS4 produces some sesquiterpenes such as δ-elemene. The RlemTPS4 gene was specifically expressed in specialized epithelial cells surrounding the oil secretory cavities in rough lemon leaf tissue.

Keywords: Citrus; Essential oil; Sesquiterpene; Terpene synthase; δ-Elemene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkyl and Aryl Transferases / chemistry
Alkyl and Aryl Transferases / genetics
Alkyl and Aryl Transferases / isolation & purification*
Alkyl and Aryl Transferases / metabolism
Amino Acid Sequence
Citrus / enzymology*
Citrus / genetics
Epithelial Cells / enzymology*
Gene Expression Regulation, Plant
Molecular Sequence Data
Plant Leaves / genetics
Plant Leaves / metabolism
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / isolation & purification*
Plant Proteins / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Analysis, Protein

Substances

Plant Proteins
RNA, Messenger
Alkyl and Aryl Transferases
terpene synthase