Abstract
A new compound designated as clonoamide was isolated from a culture broth of the fungus Clonostachys sp. BF-0131 by solvent extraction, Diaion HP20 column chromatography, octadecylsilyl column chromatography and preparative HPLC as an inhibitor of sterol O-acyltransferase (SOAT). The structure of clonoamide was elucidated as 2-oxo-9E,11E-tridecandienyl acetamide by various spectral analyses including NMR. The compound inhibited SOAT1 and SOAT2 isozymes with IC50 values of 39 and 110 μm, respectively, in a cell-based assay using SOAT1- and SOAT2-expressing Chinese hamster ovary cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetamides / chemistry*
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Acetamides / metabolism
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Animals
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Anti-Infective Agents / pharmacology
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CHO Cells
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Chlorocebus aethiops
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Cricetinae
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Cricetulus
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Enzyme Inhibitors / pharmacology*
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Fermentation
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Fungi / chemistry*
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Isoenzymes / antagonists & inhibitors
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Macrophages, Peritoneal / drug effects
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Macrophages, Peritoneal / enzymology
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Magnetic Resonance Spectroscopy
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Mice
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Microbial Sensitivity Tests
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Microsomes / drug effects
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Microsomes / enzymology
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Sterol O-Acyltransferase / antagonists & inhibitors*
Substances
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Acetamides
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Anti-Infective Agents
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Enzyme Inhibitors
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Isoenzymes
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clonoamide
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Sterol O-Acyltransferase