Methods for the characterization of stress granules in virus infected cells

Marc D Panas; Nancy Kedersha; Gerald M McInerney

doi:10.1016/j.ymeth.2015.04.009

Methods for the characterization of stress granules in virus infected cells

Methods. 2015 Nov 15:90:57-64. doi: 10.1016/j.ymeth.2015.04.009. Epub 2015 Apr 18.

Authors

Marc D Panas¹, Nancy Kedersha², Gerald M McInerney³

Affiliations

¹ Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
² Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, MA, USA.
³ Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. Electronic address: gerald.mcinerney@ki.se.

Abstract

Stress granules are induced in many different viral infections, and in turn are inhibited by the expression of viral proteins or RNAs. It is therefore evident that these bodies are not compatible with efficient viral replication, but the mechanism by which they act to restrict viral gene expression or genome replication is not yet understood. This article discusses a number of methods that can be employed to gain a more complete understanding of the relationship between cellular SGs and viral RNA and protein synthesis in cells infected with diverse viruses.

Keywords: Cell biology; Signaling; Stress granules; Translation; Virus.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Host-Pathogen Interactions
Microscopy / methods
RNA, Viral / analysis
Ribonucleoproteins / chemistry
Ribonucleoproteins / metabolism
Stress, Physiological
Viral Proteins / analysis*
Virology / methods*

Substances

RNA, Viral
Ribonucleoproteins
Viral Proteins