Abstract
A sugar epitope, α-(2,9)-trisialic acid, was synthesized and labeled by cyanine dyes through Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The cyanine tagged oligosialic acid can be utilized as an efficient fluorescent probe to image the glycan-binding receptors on PC-12 cells. The distribution of Sia-binding immunoglobulin-like lectins (Siglecs) for α-(2,9)-trisialic acid was visualized by Cy3/Cy5 or FRET channel fluorescence imaging.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carbocyanines / chemical synthesis*
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Carbocyanines / chemistry*
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Cell Survival
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Fluorescent Dyes / chemistry*
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Molecular Structure
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PC12 Cells
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Polysaccharides / metabolism*
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Rats
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Receptors, Cell Surface / analysis*
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Receptors, Cell Surface / metabolism*
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Sialic Acids / chemical synthesis*
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Sialic Acids / chemistry*
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Trisaccharides / chemical synthesis*
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Trisaccharides / chemistry*
Substances
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Carbocyanines
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Fluorescent Dyes
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Polysaccharides
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Receptors, Cell Surface
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Sialic Acids
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Trisaccharides