Sub-minute method for simultaneous determination of aspartame, cyclamate, acesulfame-K and saccharin in food and pharmaceutical samples by capillary zone electrophoresis

J Chromatogr A. 2015 May 29:1396:148-52. doi: 10.1016/j.chroma.2015.03.070. Epub 2015 Apr 8.

Abstract

This paper reports the development of a sub-minute separation method by capillary zone electrophoresis for the determination of aspartame, cyclamate, acesulfame-K and saccharin in food products and pharmaceutical samples. Separations were performed in a fused uncoated silica capillary with UV detection at 220nm. Samples and standards were injected hydrodynamically using the short-end injection procedure. The electrophoretic system was operated under constant voltage of -30kV. The background electrolyte was composed of 45mmolL(-1) 2-amino-2-(hydroxymethyl)-1,3-propanediol and 15mmolL(-1) benzoic acid at pH 8.4. The separation time for all analytes was less than 1min. Evaluation of analytical parameters of the method showed good linearity (r(2)>0.9972), limit of detection of 3.3-6.4mgL(-1), intermediate precision better than 9.75% (peak area of sample) and recovery in the range of 91-117%.

Keywords: Artificial sweetener; Capillary zone electrophoresis; Fast method; Food products; Pharmaceutical preparations.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Aspartame / analysis*
  • Cyclamates / analysis*
  • Electrolytes
  • Electrophoresis, Capillary / methods
  • Food Analysis*
  • Pharmaceutical Preparations / analysis*
  • Saccharin / analysis*
  • Sweetening Agents / analysis*
  • Thiazines / analysis*

Substances

  • Cyclamates
  • Electrolytes
  • Pharmaceutical Preparations
  • Sweetening Agents
  • Thiazines
  • Saccharin
  • acetosulfame
  • Aspartame