Backbone and side-chain (1)H, (15)N, (13)C assignment and secondary structure of BPSL1445 from Burkholderia pseudomallei

Giacomo Quilici; Andrea Berardi; Davide Gaudesi; Louise J Gourlay; Martino Bolognesi; Giovanna Musco

doi:10.1007/s12104-015-9607-1

Backbone and side-chain (1)H, (15)N, (13)C assignment and secondary structure of BPSL1445 from Burkholderia pseudomallei

Biomol NMR Assign. 2015 Oct;9(2):347-50. doi: 10.1007/s12104-015-9607-1. Epub 2015 Apr 19.

Authors

Giacomo Quilici¹, Andrea Berardi¹, Davide Gaudesi¹, Louise J Gourlay², Martino Bolognesi², Giovanna Musco³

Affiliations

¹ Biomolecular NMR Unit, c/o S. Raffaele Scientific Institute, Via Olgettina, 58, Milan, Italy.
² Department of Biosciences, University of Milan, Via Celoria, 26, 20133, Milan, Italy.
³ Biomolecular NMR Unit, c/o S. Raffaele Scientific Institute, Via Olgettina, 58, Milan, Italy. musco.giovanna@hsr.it.

PMID: 25893672
DOI: 10.1007/s12104-015-9607-1

Abstract

BPSL1445 is a lipoprotein produced by the Gram-negative bacterium Burkholderia pseudomallei (B. pseudomallei), the etiological agent of melioidosis. Immunodetection assays against sera patients using protein microarray suggest BPSL1445 involvement in melioidosis. Herein we report backbone, side chain NMR assignment and secondary structure for the recombinant protein.

Keywords: Biological warfare agent; Host-pathogen interactions; Infectious diseases; Melioidosis; Vaccine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / chemistry*
Burkholderia pseudomallei / metabolism*
Carbon-13 Magnetic Resonance Spectroscopy*
Nitrogen Isotopes
Protein Structure, Secondary
Proton Magnetic Resonance Spectroscopy*

Substances

Bacterial Proteins
Nitrogen Isotopes