Identification of Single Nucleotide Polymorphism Markers in the Laccase Gene of Shiitake Mushrooms (Lentinula edodes)

Ki-Hwan Kim; Kang-Hyeon Ka; Ji Hyoun Kang; Sangil Kim; Jung Won Lee; Bong-Kyun Jeon; Jung-Kuk Yun; Sang Rul Park; Hyuk Je Lee

doi:10.5941/MYCO.2015.43.1.75

Identification of Single Nucleotide Polymorphism Markers in the Laccase Gene of Shiitake Mushrooms (Lentinula edodes)

Mycobiology. 2015 Mar;43(1):75-80. doi: 10.5941/MYCO.2015.43.1.75. Epub 2015 Mar 31.

Authors

Ki-Hwan Kim¹, Kang-Hyeon Ka², Ji Hyoun Kang³, Sangil Kim⁴, Jung Won Lee⁵, Bong-Kyun Jeon⁵, Jung-Kuk Yun⁶, Sang Rul Park⁴, Hyuk Je Lee⁷

Affiliations

¹ Bionics, Seoul 135-757, Korea.
² Korea Forest Research Institute, Seoul 130-712, Korea.
³ Lehrstuhl für Zoologie und Evolutionsbiologie, Department of Biology, University of Konstanz, Konstanz 78457, Germany.
⁴ Estuarine & Coastal Ecology Laboratory, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea.
⁵ Smarteome Co., Suwon 441-853, Korea.
⁶ Department of Medicine, Graduate School, Chungbuk National University, Cheongju 362-763, Korea.
⁷ Department of Biological Science, College of Science and Engineering, Sangji University, Wonju 220-702, Korea.

Abstract

We identified single nucleotide polymorphism (SNP) markers in the laccase gene to establish a line-diagnostic system for shiitake mushrooms. A total of 89 fungal isolates representing four lines, including Korean registered, Korean wild type, Chinese, and Japanese lines, were analyzed. The results suggest that SNP markers in the laccase gene can be useful for line typing in shiitake mushrooms.

Keywords: Laccase gene; Lentinula edodes; Mushroom; SNP; Shiitake.