The critical glucose concentration for respiration-independent proliferation of fission yeast, Schizosaccharomyces pombe

Kojiro Takeda; Caroline Starzynski; Ayaka Mori; Mitsuhiro Yanagida

doi:10.1016/j.mito.2015.04.003

The critical glucose concentration for respiration-independent proliferation of fission yeast, Schizosaccharomyces pombe

Mitochondrion. 2015 May:22:91-5. doi: 10.1016/j.mito.2015.04.003. Epub 2015 Apr 17.

Authors

Kojiro Takeda¹, Caroline Starzynski², Ayaka Mori², Mitsuhiro Yanagida²

Affiliations

¹ G0 Cell Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Okinawa 904-1111, Japan. Electronic address: takeda@center.konan-u.ac.jp.
² G0 Cell Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna, Okinawa 904-1111, Japan.

PMID: 25891397
DOI: 10.1016/j.mito.2015.04.003

Abstract

Glucose is the fundamental energy source for life; thus cells need to respond appropriately to changes in available glucose concentration. We investigated the relationship between media glucose concentration and respiration-dependency of proliferation, using Schizosaccharomyces pombe. In media containing ≥ 0.2% glucose, neither antimycin A, an inhibitor of Complex III, nor gene deletions of essential electron transfer chain components, impaired cell division, while these factors completely inhibited cell division in media containing ≤ 0.1% glucose. These results indicate the existence of a threshold in glucose concentration that governs respiration-dependency of S. pombe proliferation.

Keywords: Antimycin A; Cell cycle; Fission yeast; Glucose concentration threshold; Respiration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aerobiosis
Anaerobiosis
Energy Metabolism
Glucose / metabolism*
Oxygen / metabolism*
Schizosaccharomyces / growth & development*
Schizosaccharomyces / metabolism*

Substances

Glucose
Oxygen