Post-translational modifications (PTMs) play an important role in life science. The most widely studied protein modifications in biological science include protein phosphorylation, acylation, glycosylation, ubiquitination, acetylation, oxidation, methylation and so on. This review outlines current achievements in the study of protein modifications in muscle food using proteomic approaches. First we describe the general knowledge of protein modifications and then the development of proteomic approaches for the characterization of such modifications. Second, we describe the effects of protein modifications on muscle foods and devote our main attention to the application of proteomic approaches for the analysis of these modifications. We conclude that proteomics analysis is powerful for the study of protein modifications and analysis of meat quality characteristics in the process of food production.
蛋白质翻译后修饰(Post-translational modifications, PTMs)在生命体中具有十分重要的作用。生命有机体中常见的PTMs有磷酸化、酰化、糖基化、泛素化、乙酰化、氧化和甲基化等。文章主要介绍了蛋白质组学在肉制品科学方面的应用、PTMs的主要内容以及分析蛋白修饰特性常见技术的发展,总结了PTMs对肌肉生理特性的影响和蛋白质组学方法在肉质蛋白质修饰研究中的重要性及前景,讨论了利用蛋白质修饰组学技术研究肌肉熟化过程中品质特性变化的特点。.