Abstract
Environmental contamination by petroleum hydrocarbons, mainly crude oil waste from refineries, is becoming prevalent worldwide. This study investigates the bioremediation of water contaminated with crude oil waste. Bacillus salamalaya 139SI, a bacterium isolated from a private farm soil in the Kuala Selangor in Malaysia, was found to be a potential degrader of crude oil waste. When a microbial population of 108 CFU ml-1 was used, the 139SI strain degraded 79% and 88% of the total petroleum hydrocarbons after 42 days of incubation in mineral salt media containing 2% and 1% of crude oil waste, respectively, under optimum conditions. In the uninoculated medium containing 1% crude oil waste, 6% was degraded. Relative to the control, the degradation was significantly greater when a bacteria count of 99 × 108 CFU ml-1 was added to the treatments polluted with 1% oil. Thus, this isolated strain is useful for enhancing the biotreatment of oil in wastewater.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacillus / genetics
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Bacillus / growth & development
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Bacillus / isolation & purification
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Bacillus / metabolism*
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Biodegradation, Environmental
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Emulsifying Agents / metabolism
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Hemolysis
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Industrial Microbiology / methods*
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Petroleum / analysis*
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Petroleum / microbiology*
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Phylogeny
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Sheep
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Soil Microbiology
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Surface-Active Agents / metabolism
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Waste Disposal, Fluid / methods
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Water Pollution, Chemical / analysis*
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Water Purification / methods*
Substances
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Emulsifying Agents
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Petroleum
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Surface-Active Agents
Grants and funding
The authors would like to acknowledge the support of chancellery of University Malaya with special grants numbered A-21010-DA674 and A-21010-DA677. Also, the authors would like to acknowledge the support of the University of Malaya Research Program (UMRP) with grant number RP023A-14AFR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.