A molecular imprinted SPR biosensor for sensitive determination of citrinin in red yeast rice

Food Chem. 2015 Oct 1:184:7-11. doi: 10.1016/j.foodchem.2015.03.065. Epub 2015 Mar 27.

Abstract

A novel and sensitive molecular imprinted surface plasmon resonance (SPR) biosensor was developed for selective determination of citrinin (CIT) in red yeast rice. Firstly, the gold surface of SPR chip was modified with allyl mercaptane. Then, CIT-imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) (p(HEMA-MAGA)) film was generated on the gold surface modified with allyl mercaptane. The unmodified and imprinted surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and contact angle measurements. The linearity range and the detection limit were obtained as 0.005-1.0 ng/mL and 0.0017 ng/mL, respectively. The SPR biosensor was applied to determination of CIT in red yeast rice sample.

Keywords: Biosensor; Food analysis; Mycotoxin; Red yeast rice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Products / chemistry*
  • Biosensing Techniques / methods*
  • Citrinin / analysis*
  • Limit of Detection
  • Microscopy, Atomic Force
  • Molecular Imprinting / methods*
  • Spectroscopy, Fourier Transform Infrared
  • Surface Plasmon Resonance / methods*

Substances

  • Biological Products
  • red yeast rice
  • Citrinin