Simple and Sensitive Point-of-Care Bioassay System Based on Hierarchically Structured Enzyme-Mimetic Nanoparticles

Miju Kim; Min Su Kim; Soon Ho Kweon; Sanha Jeong; Mi Hyun Kang; Moon Il Kim; Jinwoo Lee; Junsang Doh

doi:10.1002/adhm.201500173

Simple and Sensitive Point-of-Care Bioassay System Based on Hierarchically Structured Enzyme-Mimetic Nanoparticles

Adv Healthc Mater. 2015 Jun 24;4(9):1311-6. doi: 10.1002/adhm.201500173. Epub 2015 Apr 11.

Authors

Miju Kim^{1

2}, Min Su Kim³, Soon Ho Kweon², Sanha Jeong³, Mi Hyun Kang⁴, Moon Il Kim⁵, Jinwoo Lee³, Junsang Doh^{1

2}

Affiliations

¹ Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 790-784, Republic of Korea.
² School of Interdisciplinary Bioscience and Bioengineering (I-Bio), POSTECH, Pohang, Gyeongbuk, 790-784, Republic of Korea.
³ Department of Chemical Engineering, POSTECH, Pohang, Gyeongbuk, 790-784, Republic of Korea.
⁴ Department of Nano Medical Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
⁵ Department of BioNano Technology, Gachon University, Seongnam, Gyeonggi, 461-701, Republic of Korea.

PMID: 25866283
DOI: 10.1002/adhm.201500173

Abstract

An enzyme-mimetic nanoparticle-based point of care bioassay device is developed for rapid and sensitive detection of analytes. Digital images acquired by smart cellular phones allow quantifying the amounts of analytes. Using this new device, quantitative analysis of liquid sample is performed within 15 min with an order of magnitude enhancement of sensitivity compared with conventional Au nanoparticle-based devices.

Keywords: biosensors; diagnostics; enzyme-mimetics; immunoassays; nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biological Assay / instrumentation
Biological Assay / methods*
Biomimetic Materials / chemistry*
Humans
Nanoparticles / chemistry*
Peroxidase / chemistry*
Point-of-Care Systems*

Substances

Peroxidase