Protective immune responses in ducklings induced by a suicidal DNA vaccine of the sigma C gene of novel duck reovirus

Yingqi Zhu; Chuanfeng Li; Zhuangli Bi; Zongyan Chen; Chunchun Meng; Gui-Jun Wang; Chan Ding; Guangqing Liu

doi:10.1016/j.vetimm.2015.03.001

Protective immune responses in ducklings induced by a suicidal DNA vaccine of the sigma C gene of novel duck reovirus

Vet Immunol Immunopathol. 2015 May 15;165(1-2):88-92. doi: 10.1016/j.vetimm.2015.03.001. Epub 2015 Mar 6.

Authors

Yingqi Zhu¹, Chuanfeng Li², Zhuangli Bi¹, Zongyan Chen², Chunchun Meng², Gui-Jun Wang³, Chan Ding², Guangqing Liu⁴

Affiliations

¹ Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
² Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China.
³ College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, PR China.
⁴ Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241 Shanghai, PR China. Electronic address: liugq@shvri.ac.cn.

PMID: 25863968
DOI: 10.1016/j.vetimm.2015.03.001

Abstract

A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against novel duck reovirus (NDRV). The sigma C gene of NDRV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. After transfected into BHK-21 cells, the antigenicity of the sigma C protein was confirmed using an indirect immunofluorescence and Western blot assay. Immunogenicity was studied in ducklings. The results showed that NDRV-specific antibodies, neutralizing antibodies, IFN-γ and IL-4 were well induced in ducklings. Furthermore, all of the ducklings were protected against challenge with wild-type NDRV.

Keywords: Novel duck reovirus; Sigma C gene; Suicidal DNA vaccine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Neutralizing / immunology
Antibodies, Viral / immunology
Blotting, Western / veterinary
Ducks / immunology*
Fluorescent Antibody Technique, Indirect / veterinary
Interferon-gamma / blood
Interleukin-4 / blood
Poultry Diseases / immunology
Poultry Diseases / prevention & control
Poultry Diseases / virology*
Reoviridae / immunology*
Reoviridae Infections / immunology
Reoviridae Infections / prevention & control
Reoviridae Infections / veterinary*
Vaccines, DNA / immunology
Vaccines, DNA / pharmacology*
Viral Vaccines / immunology
Viral Vaccines / pharmacology*

Substances

Antibodies, Neutralizing
Antibodies, Viral
Vaccines, DNA
Viral Vaccines
Interleukin-4
Interferon-gamma