Assessment of white grape pomace from winemaking as source of bioactive compounds, and its antiproliferative activity

M José Jara-Palacios; Dolores Hernanz; Tania Cifuentes-Gomez; M Luisa Escudero-Gilete; Francisco J Heredia; Jeremy P E Spencer

doi:10.1016/j.foodchem.2015.03.022

Assessment of white grape pomace from winemaking as source of bioactive compounds, and its antiproliferative activity

Food Chem. 2015 Sep 15:183:78-82. doi: 10.1016/j.foodchem.2015.03.022. Epub 2015 Mar 13.

Authors

M José Jara-Palacios¹, Dolores Hernanz², Tania Cifuentes-Gomez³, M Luisa Escudero-Gilete¹, Francisco J Heredia⁴, Jeremy P E Spencer³

Affiliations

¹ Food Colour and Quality Laboratory, Department of Nutrition and Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
² Department of Analytical Chemistry, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.
³ Molecular Nutrition Group, Department of Food and Nutritional Sciences, School of Chemistry, Food and Pharmacy, University of Reading, Reading RG6 6AP, United Kingdom.
⁴ Food Colour and Quality Laboratory, Department of Nutrition and Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain. Electronic address: heredia@us.es.

PMID: 25863613
DOI: 10.1016/j.foodchem.2015.03.022

Abstract

The antiproliferative effects of a purified white grape pomace extract (PWGPE), as well as of some phenolic standards on colon cancer cells were examined. The phenolic composition of the PWGPE was determined by rapid resolution liquid chromatography/mass spectrometry (RRLC/MS). The PWGPE had 92.6, 43.3 and 6.01mg/g of flavanols, flavonols and phenolic acids, respectively and, along with pure catechin, epicatechin, quercetin and gallic acid, they were all found capable of inhibiting cellular proliferation. PWGPE (100μg/ml) inhibited the proliferation of cells by 52.1% at 48h, whilst catechin, epicatechin, quercetin and gallic acid (60μg/ml) inhibited growth by 65.2%, 62.2%, 81.0% and 71.0%, respectively, at 72h. The PWGPE is an interesting source of phenolic compounds with antiproliferative properties, that could be of interest in the food and pharmaceutical industries.

Keywords: Caco-2; Colorectal cancer; Grape pomace; Phenolic compounds.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catechin / analysis
Chromatography, High Pressure Liquid / methods*
Colorectal Neoplasms / prevention & control*
Flavonoids / analysis*
Flavonols / analysis
Humans
Hydroxybenzoates / chemistry*
Mass Spectrometry / methods*
Plant Extracts / chemistry*
Quercetin / analysis
Vitis / chemistry*

Substances

Flavonoids
Flavonols
Hydroxybenzoates
Plant Extracts
Catechin
Quercetin
phenolic acid