Harmonization of PFGE profile analysis by using bioinformatics tools: example of the Listeria monocytogenes European Union Reference Laboratory network

Benjamin Félix; Sophie Roussel; Bruno Pot

doi:10.1007/978-1-4939-2599-5_2

Harmonization of PFGE profile analysis by using bioinformatics tools: example of the Listeria monocytogenes European Union Reference Laboratory network

Methods Mol Biol. 2015:1301:9-28. doi: 10.1007/978-1-4939-2599-5_2.

Authors

Benjamin Félix¹, Sophie Roussel, Bruno Pot

Affiliation

¹ European Union Reference Laboratory for Listeria monocytogenes, ANSES, Maisons-Alfort Laboratory for Food Safety, University Paris-Est, 23 Avenue du Général De Gaulle, 94706, Maisons-Alfort, France.

PMID: 25862044
DOI: 10.1007/978-1-4939-2599-5_2

Abstract

Standardization in comparison and interpretation of profiles is an integral part of the analysis of gels from pulsed-field gel electrophoresis. Using Listeria monocytogenes as an example, this chapter outlines the analytical process using the software BioNumerics.

MeSH terms

Bacterial Typing Techniques / methods*
Computational Biology*
Electrophoresis, Gel, Pulsed-Field / methods*
European Union
Food Microbiology
Humans
Listeria monocytogenes / genetics*
Listeria monocytogenes / isolation & purification
Listeria monocytogenes / pathogenicity