Enzyme immunoassay for the detection of porcine gelatine in edible bird's nests

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2015;32(7):1023-8. doi: 10.1080/19440049.2015.1039605. Epub 2015 Apr 29.

Abstract

Porcine gelatine is a common adulterant found in edible bird's nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 µg g(-1), respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng µg(-1) (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry.

Keywords: ELISA; adulterant; edible bird’s nest; gelatine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Birds* / metabolism
  • Food Analysis / methods*
  • Food Contamination*
  • Gelatin / chemistry
  • Gelatin / classification*
  • Immunoenzyme Techniques / methods*
  • Species Specificity
  • Swine

Substances

  • Gelatin