A Genetically Encoded FRET Sensor for Intracellular Heme

Yanqun Song; Maiyun Yang; Seraphine V Wegner; Jingyi Zhao; Rongfeng Zhu; Yun Wu; Chuan He; Peng R Chen

doi:10.1021/cb5009734

A Genetically Encoded FRET Sensor for Intracellular Heme

ACS Chem Biol. 2015 Jul 17;10(7):1610-5. doi: 10.1021/cb5009734. Epub 2015 Apr 17.

Authors

Yanqun Song¹, Maiyun Yang¹, Seraphine V Wegner^{1

2}, Jingyi Zhao³, Rongfeng Zhu⁴, Yun Wu⁵, Chuan He^{1

2

6}, Peng R Chen^{1

4

6}

Affiliations

¹ †Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
² ‡Department of Chemistry and Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.
³ §Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University, Shenzhen 518055, China.
⁴ ∥Peking-Tinghua Center for Life Sciences, Beijing, China.
⁵ ⊥State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
⁶ #Shanghai Universities E-Institute for Chemical Biology, Shanghai, China.

PMID: 25860383
DOI: 10.1021/cb5009734

Abstract

Heme plays pivotal roles in various cellular processes as well as in iron homeostasis in living systems. Here, we report a genetically encoded fluorescence resonance energy transfer (FRET) sensor for selective heme imaging by employing a pair of bacterial heme transfer chaperones as the sensory components. This heme-specific probe allows spatial-temporal visualization of intracellular heme distribution within living cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacillus anthracis / genetics
Bacillus anthracis / metabolism
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Biosensing Techniques / methods*
Cell Line
Cell Line, Tumor
Cricetinae
Fluorescence Resonance Energy Transfer / methods*
Genetic Engineering
Heme / analysis*
Heme / metabolism
Humans
Luminescent Proteins / genetics
Luminescent Proteins / metabolism
Mice
Optical Imaging / methods

Substances

Bacterial Proteins
Luminescent Proteins
Heme

Grants and funding

Howard Hughes Medical Institute/United States