Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu(2+) ions

Willer Ferreira da Silva Júnior; Rodrigo Cano; Antonio Helvécio Totola; Lorendane Milena de Carvalho; Marcel Otávio Cerri; Jane Sélia Dos Reis Coimbra; Gleidson Giordano Pinto de Carvalho; Bruna Mara Aparecida de Carvalho

doi:10.1016/j.chroma.2015.03.052

Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu(2+) ions

J Chromatogr A. 2015 May 22:1395:16-22. doi: 10.1016/j.chroma.2015.03.052. Epub 2015 Mar 27.

Authors

Willer Ferreira da Silva Júnior¹, Rodrigo Cano¹, Antonio Helvécio Totola¹, Lorendane Milena de Carvalho², Marcel Otávio Cerri³, Jane Sélia Dos Reis Coimbra⁴, Gleidson Giordano Pinto de Carvalho⁵, Bruna Mara Aparecida de Carvalho⁶

Affiliations

¹ Department of Chemistry, Biotechnology and Bioprocess Engineering, Federal University of São João Del Rei, Ouro Branco, MG 36420-000, Brazil.
² Department of Veterinary, Federal University of Viçosa, Viçosa, MG 36570-000, Brazil.
³ Department of Bioprocess and Biotechnology, São Paulo State University, Araraquara, SP, 14801-902, Brazil.
⁴ Department of Food Technology, Federal University of Viçosa, Viçosa, MG 36570-000, Brazil.
⁵ Department of Animal Science, Federal University of Bahia, Salvador, BA 40110-909, Brazil.
⁶ Institute of Agricultural Sciences, Federal University of Minas Gerais, Montes Claros, MG 39400-401, Brazil. Electronic address: brunamara.carvalho@gmail.com.

PMID: 25857540
DOI: 10.1016/j.chroma.2015.03.052

Abstract

The influence of temperature, pH and ionic strength on the adsorption of Immunoglobulin Y (IgY) in IDA-Cu(2+) cryogel system was studied by batch equilibrium measurements. The adsorptive equilibrium data were obtained at 17 and 27°C, pH 5.0 and 6.5, and at ionic strength of 50 and 200mmolL(-1) NaCl. Langmuir, Freundlich and Langmuir-Freundlich models were fitted to equilibrium data, while the enthalpy of adsorption of IgY in IDA-Cu(2+) cryogel system was calculated through Van't Hoff analysis. The binding of IgY on cryogel was stronger at 27°C and lowest pH and ionic strength values, with apparent maximum adsorption capacity of 27mgg(-1). The adsorption of protein in the resin was spontaneous in all analyzed cases. The results provide valuable information to enable the improvement of IgY purification processes.

Keywords: Adsorption isotherms; Cryogel; IMAC; Immunoglobulin Y; Thermodynamic parameters.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Biotechnology / methods*
Copper / chemistry*
Cryogels / chemistry*
Hydrogen-Ion Concentration
Immunoglobulins / isolation & purification*
Ions / chemistry
Osmolar Concentration
Proteins
Sodium Chloride
Temperature
Thermodynamics

Substances

Cryogels
IgY
Immunoglobulins
Ions
Proteins
Sodium Chloride
Copper