Genomic characterization of EmsB microsatellite loci in Echinococcus multilocularis

Infect Genet Evol. 2015 Jun:32:338-41. doi: 10.1016/j.meegid.2015.03.040. Epub 2015 Apr 4.

Abstract

EmsB is a molecular marker applied to Echinococcus multilocularis genotyping studies. This marker has largely been used to investigate the epidemiology of the parasite in different endemic foci. The present study has lifted the veil on the genetic structure of this microsatellite. By in silico analysis on the E. multilocularis genome the microsatellite was described in about 40 copies on the chromosome 5 of the parasite. Similar structure was found in the relative parasite Echinococcus granulosus, where the microsatellite was firstly described. The present study completes the first investigations made on the EmsB microsatellite origins and confirms the reliability of this highly discriminant molecular marker.

Keywords: Echinococcus multilocularis; EmsB microsatellite; Genetic diversity; Genome; In silico analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA, Helminth / genetics
  • Echinococcus granulosus / classification
  • Echinococcus granulosus / genetics
  • Echinococcus multilocularis / classification
  • Echinococcus multilocularis / genetics*
  • Genetic Loci*
  • Genome, Helminth*
  • Genomics
  • Genotyping Techniques
  • Helminth Proteins / genetics*
  • Microsatellite Repeats*
  • Molecular Sequence Data
  • Reproducibility of Results

Substances

  • DNA, Helminth
  • Helminth Proteins