Synthesis and characterization of nanosilver with antibacterial properties using Pinus densiflora young cone extract

J Photochem Photobiol B. 2015 Jun:147:63-8. doi: 10.1016/j.jphotobiol.2015.03.008. Epub 2015 Mar 23.

Abstract

This study describes an eco-friendly, rapid method for green synthesis of silver nanoparticles (Ag NPs) from an aqueous solution of silver nitrate using Pinus densiflora for. multicaulis Uyeki young cone extract in a single-pot process. Color changes, ultraviolet-visible spectra (444.5 nm), X-ray diffraction peaks (2θ=39.68, 46.92, 68.12, and 79.10), and Fourier transform infrared spectroscopy (FT-IR) confirmed the presence of Ag NPs and phytochemicals. Transmission electron microscopy showed that the nanoparticles were mostly oval in shape, with a few triangular-shaped particles. Average particle size was 30-80 nm. Phytochemicals present in the young pine cone extract were likely responsible for the reduction of Ag(+) ions. The synthesized Ag NPs (40 μg) had a 7 mm larger zone of inhibition against the skin pathogen Brevibacterium linens than commercial Ag NPs, Propionibacterium acnes (14 mm), Bacillus cereus (9 mm) and Staphylococcus epidermidis (10mm).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / chemical synthesis
  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology*
  • Bacillus cereus / drug effects
  • Chemistry Techniques, Synthetic
  • Drug Evaluation, Preclinical / methods
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / ultrastructure
  • Microscopy, Electron, Transmission
  • Pinus / chemistry*
  • Plant Extracts / chemistry*
  • Propionibacterium acnes / drug effects
  • Silver / chemistry
  • Silver / pharmacology*
  • Spectrophotometry, Ultraviolet
  • Spectroscopy, Fourier Transform Infrared
  • Staphylococcus epidermidis / drug effects
  • X-Ray Diffraction

Substances

  • Anti-Bacterial Agents
  • Plant Extracts
  • Silver