Abstract
We previously found that miR-29a was significantly downregulated in Ankylosing spondylitis (AS) patients, a chronic inflammatory disease associated with bone metabolic disorder, however, the underlying mechanism remains unclear. In this study, we demonstrated that miR-29a regulates tumor necrosis factor-α (TNF-α) mediated bone loss mainly by targeting DKK1 and GSK3β, thus activating the Wnt/β-catenin pathway. Our findings may provide new insight into the pathogenesis of the bone metabolism disorder in inflammation environment and provide promising therapeutic target.
Keywords:
TNF-α; Wnt antagonists; miR-29a.
© 2015 Japanese Society of Developmental Biologists.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anthraquinones
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Blotting, Western
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Bone Resorption / metabolism*
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Glycogen Synthase Kinase 3 / metabolism
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Glycogen Synthase Kinase 3 beta
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Humans
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Intercellular Signaling Peptides and Proteins / metabolism
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Luciferases
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MicroRNAs / metabolism*
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Oligonucleotides / genetics
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Real-Time Polymerase Chain Reaction
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Spondylitis, Ankylosing / metabolism*
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Tumor Necrosis Factor-alpha / metabolism*
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Wnt Signaling Pathway / physiology*
Substances
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Anthraquinones
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DKK1 protein, human
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Intercellular Signaling Peptides and Proteins
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MIRN29a microRNA, human
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MicroRNAs
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Oligonucleotides
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Tumor Necrosis Factor-alpha
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alizarin
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Luciferases
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GSK3B protein, human
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Glycogen Synthase Kinase 3 beta
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Glycogen Synthase Kinase 3