Cellular aggregate capture by fluidic manipulation device highly compatible with micro-well-plates

Biomed Microdevices. 2015;17(3):9953. doi: 10.1007/s10544-015-9953-x.

Abstract

This paper proposes a capture device to manipulate and transport a cellular aggregate in a micro-well. A cellular aggregate (a few hundreds μm in diameter) is currently manipulated by a pipette. The manual manipulation by a pipette has problems; low reliability, low throughput, and difficulty in confirmation of task completion. We took into account of compatibility with existing methods such as a micro-well-plate and designed for the capture device of a cellular aggregate in a micro-well. A newly developed capture device flows and carries a cellular aggregate from a bottom of a well to a trap of the capture device. We designed a curved surface at the bottom of the capture device to form a space to act as a channel between the inner wall of the micro-well. This paper presents concept, design, fabrication, and of the proposed cellular aggregate capture, followed by successful experimental results.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Aggregation / physiology
  • Cell Culture Techniques / instrumentation
  • Cell Separation / instrumentation*
  • Cells, Cultured
  • Equipment Design
  • Equipment Failure Analysis
  • Flow Cytometry / instrumentation*
  • Humans
  • Lab-On-A-Chip Devices*
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / physiology
  • Micromanipulation / instrumentation*
  • Specimen Handling / instrumentation