For the first time, a simple, ultrasensitive and label-free electrochemical monocyte chemotactic protein-1 (MCP-1) immunosensor based on orderly oriented conductive wires has been developed. A conductive wire, which is similar to an electron-conducting tunnel, was designed with Au nanoparticles (AuNPs) joined to Au@Pt core-shell microspheres via a cysteamine (CA) crosslinker. To enhance the sensitivity of the immunosensor, Au nanoparticles were electrodeposited onto the gold electrode, and CA was self-assembled via strong Au-S covalent bonds, providing an appropriate surface and promoting electron transfer. Next, Au@Pt core-shell microspheres with large surface area were grafted onto the modified electrode to immobilize more MCP-1 antibodies. MCP-1 is an initiating factor and biomarker of atherosclerotic diseases. Under optimal experimental conditions, differential pulse voltammetry (DPV) current changes were used to detect MCP-1 with a broad linear range of 0.09-360 pg mL(-1) and a low detection limit of 0.03 pg mL(-1) (S/N=3). The proposed immunosensor exhibited good selectivity, reproducibility and reusability. When applied to spiked serum samples, the data for the developed immunosensor were in agreement with an enzyme linked immunosorbent assay, suggesting that the electrochemical immunosensor would be suitable for practical detection.
Keywords: Atherosclerotic disease; Au electrodeposition; Au@Pt core–shell microspheres; Cysteamine; Monocyte chemotactic protein-1.
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