The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity

Carbohydr Polym. 2015 Jun 5:123:115-21. doi: 10.1016/j.carbpol.2015.01.028. Epub 2015 Jan 21.

Abstract

The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy. The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected. The Y-LPS-Ch complexes had similar positive ζ-potentials regardless of Ch content. The transformation of the supramolecular structure of E-LPS after binding with to Ch was revealed. Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected. The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied. A significant decrease in activity complexes compared to that of the initial LPS was observed only for E-LPS-Ch complexes.

Keywords: Chitosan; Chitosan (PubChem CID: 71853); LPS–chitosan complexes; Lipopolysaccharide (LPS); Lipopolysaccharide (PubChem CID: 11970143); Supramolecular structure; ζ-Potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chitosan / chemistry*
  • Chitosan / pharmacology
  • Escherichia coli
  • Humans
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / pharmacology
  • Microscopy, Atomic Force
  • Neutrophils / drug effects
  • Neutrophils / metabolism
  • Reactive Oxygen Species / metabolism
  • Surface Properties
  • Tumor Necrosis Factor-alpha / metabolism
  • Yersinia pseudotuberculosis

Substances

  • Lipopolysaccharides
  • Reactive Oxygen Species
  • Tumor Necrosis Factor-alpha
  • Chitosan