Determination of creatinine, uric and ascorbic acid in bovine milk and orange juice by hydrophilic interaction HPLC

Ruiting Zuo; Si Zhou; Yuegang Zuo; Yiwei Deng

doi:10.1016/j.foodchem.2015.02.142

Determination of creatinine, uric and ascorbic acid in bovine milk and orange juice by hydrophilic interaction HPLC

Food Chem. 2015 Sep 1:182:242-5. doi: 10.1016/j.foodchem.2015.02.142. Epub 2015 Mar 6.

Authors

Ruiting Zuo¹, Si Zhou², Yuegang Zuo³, Yiwei Deng⁴

Affiliations

¹ Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747, USA; College of Literature, Science, and the Arts, University of Michigan, 500 S. State St., Ann Arbor, MI 48109, USA.
² Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747, USA.
³ Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747, USA. Electronic address: yzuo@umassd.edu.
⁴ Natural Sciences Department, University of Michigan-Dearborn, Dearborn, MI 48128, USA.

PMID: 25842333
DOI: 10.1016/j.foodchem.2015.02.142

Abstract

Creatinine (Cr), uric (UA) and ascorbic acid (AA) are common constituents in human fluids. Their abnormal concentrations in human fluids are associated with various diseases. Thus, apart from the endogenous formation in human body, it is also important to examine their sources from food products. In this study, a rapid and accurate HILIC method was developed for simultaneous determination of Cr, UA and AA in bovine milk and orange juice. Milk samples were pretreated by protein precipitation, centrifugation and filtration, followed by HPLC separation and quantification using a Waters Spherisorb S5NH2 column. The developed method has been successfully applied to determine the concentration of UA, AA and Cr in milk and fruit juice samples. The milk samples tested were found to contain UA and creatinine in the concentration range of 24.1-86.0 and 5.07-11.2 μg mL(-1), respectively. The orange juices contain AA over 212 μg mL(-1).

Keywords: Ascorbic acid; Creatinine; Hydrophilic interaction liquid chromatography (HILIC); Milk; Orange juice; Uric acid; Vitamin C.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ascorbic Acid / analysis
Ascorbic Acid / chemistry*
Beverages / analysis*
Cattle
Chromatography, High Pressure Liquid / methods*
Citrus sinensis / chemistry*
Creatinine / chemistry*
Dairy Products
Humans
Milk / chemistry*
Uric Acid / chemistry*

Substances

Uric Acid
Creatinine
Ascorbic Acid