Protective effects of bacterial osmoprotectant ectoine on bovine erythrocytes subjected to staphylococcal alpha-haemolysin

Toxicon. 2015 Jun 1:99:130-5. doi: 10.1016/j.toxicon.2015.03.022. Epub 2015 Apr 1.

Abstract

Ectoine (ECT) is a bacterial compatible solute with documented protective action however no data are available on its effects on various cells against bacterial toxins. Therefore, we determined the in vitro influence of ECT on bovine erythrocytes subjected to staphylococcal α-haemolysin (HlyA). The cells exposed to HlyA alone showed a distinct haemolysis and reduced glutathione (GSH)/oxidised glutathione (GSSG) level, however the toxic effects were attenuated in the combinations of HlyA + ECT suggesting ECT-induced protection of erythrocytes from HlyA.

Keywords: Alpha-haemolysin; Bovine erythrocytes; Compatible solutes; Ectoine; Osmoprotectant.

MeSH terms

  • Amino Acids, Diamino / pharmacology*
  • Animals
  • Bacterial Toxins / antagonists & inhibitors*
  • Bacterial Toxins / toxicity
  • Cattle
  • Cryoprotective Agents / pharmacology*
  • Erythrocytes / drug effects*
  • Exotoxins / antagonists & inhibitors*
  • Exotoxins / toxicity
  • Glutathione / chemistry
  • Glutathione / metabolism
  • Hemolysin Proteins / antagonists & inhibitors*
  • Hemolysin Proteins / toxicity
  • Hemolysis / drug effects*
  • Hemolytic Agents / chemistry
  • Hemolytic Agents / toxicity*
  • Kinetics
  • Oxidation-Reduction
  • Oxidative Stress / drug effects
  • Poland

Substances

  • Amino Acids, Diamino
  • Bacterial Toxins
  • Cryoprotective Agents
  • Exotoxins
  • Hemolysin Proteins
  • Hemolytic Agents
  • staphylococcal alpha-toxin
  • ectoine
  • Glutathione