Abstract
Ectoine (ECT) is a bacterial compatible solute with documented protective action however no data are available on its effects on various cells against bacterial toxins. Therefore, we determined the in vitro influence of ECT on bovine erythrocytes subjected to staphylococcal α-haemolysin (HlyA). The cells exposed to HlyA alone showed a distinct haemolysis and reduced glutathione (GSH)/oxidised glutathione (GSSG) level, however the toxic effects were attenuated in the combinations of HlyA + ECT suggesting ECT-induced protection of erythrocytes from HlyA.
Keywords:
Alpha-haemolysin; Bovine erythrocytes; Compatible solutes; Ectoine; Osmoprotectant.
Copyright © 2015 Elsevier Ltd. All rights reserved.
MeSH terms
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Amino Acids, Diamino / pharmacology*
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Animals
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Bacterial Toxins / antagonists & inhibitors*
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Bacterial Toxins / toxicity
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Cattle
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Cryoprotective Agents / pharmacology*
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Erythrocytes / drug effects*
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Exotoxins / antagonists & inhibitors*
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Exotoxins / toxicity
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Glutathione / chemistry
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Glutathione / metabolism
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Hemolysin Proteins / antagonists & inhibitors*
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Hemolysin Proteins / toxicity
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Hemolysis / drug effects*
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Hemolytic Agents / chemistry
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Hemolytic Agents / toxicity*
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Kinetics
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Oxidation-Reduction
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Oxidative Stress / drug effects
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Poland
Substances
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Amino Acids, Diamino
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Bacterial Toxins
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Cryoprotective Agents
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Exotoxins
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Hemolysin Proteins
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Hemolytic Agents
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staphylococcal alpha-toxin
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ectoine
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Glutathione