Anti-platelet aggregation activities of different fractions in leaves of Apocynum venetum L

Abstract

Ethnopharmacological relevance Apocynum venetum: is widely used in Uygur and traditional Chinese medicine. Modern pharmaceutical studies have shown that leaves of A. venetum have effects of liver protection, antidepressant and regulation of blood pressure. However, it is unclear that which components have pharmacological activities.

Aim of the study: The aim was to study chemical constituents of A. venetum and its anti-platelet aggregation activity.

Materials and methods: Nephelometery was applied to evaluate anti-platelet aggregation activity of multi-components of A. venetum. Systematic separation components were characterized by HPLC analysis method, and in vitro screening active components by anti-platelet aggregation study.

Results: Ethyl acetate fraction (L-III) and L-III-4 have better anti-platelet aggregation activity than other fractions. The results indicated that isoquercitrin, hyperoside and other flavonoids have anti-platelet aggregation activity in A. venetum.

Conclusion: Our studies provide basis on the endeavors of screening chemicals with that anti-platelet aggregation activity in leaves of A. venetum.

Keywords: Anti-platelet aggregation; Apocynum venetum L.; Multi-components.