Abstract
Clearing methods that render the brain optically transparent allow high-resolution three-dimensional (3D) imaging of neural networks. We used diffusion tensor imaging (DTI) and two-photon imaging of cleared brains to analyze white matter in BTBR mice. We confirmed corpus callosum agenesis and identified an abnormal commissure close to the third ventricle. DTI and cleared-brain two-photon imaging revealed that these commissural fibers constituted a frontal clustering of the ventral hippocampal commissure and provided a detailed assessment of white matter structure in mice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agenesis of Corpus Callosum / pathology*
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Animals
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Brain / abnormalities
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Brain / anatomy & histology
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Brain / pathology*
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Corpus Callosum / pathology
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Diffusion Magnetic Resonance Imaging / methods*
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Diffusion Tensor Imaging / methods*
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Disease Models, Animal
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Fornix, Brain / pathology
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Hippocampus / pathology
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Histocytological Preparation Techniques / methods
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Image Processing, Computer-Assisted / methods
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Imaging, Three-Dimensional / methods
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Inbred Strains
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Microtomy / methods
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Nerve Net / pathology
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Third Ventricle / pathology
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Tissue Fixation / methods
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White Matter / pathology