Low molecular weight chitosan-insulin polyelectrolyte complex: characterization and stability studies

Zakieh I Al-Kurdi; Babur Z Chowdhry; Stephen A Leharne; Mahmoud M H Al Omari; Adnan A Badwan

doi:10.3390/md13041765

Low molecular weight chitosan-insulin polyelectrolyte complex: characterization and stability studies

Mar Drugs. 2015 Mar 30;13(4):1765-84. doi: 10.3390/md13041765.

Authors

Zakieh I Al-Kurdi^{1

2}, Babur Z Chowdhry³, Stephen A Leharne⁴, Mahmoud M H Al Omari⁵, Adnan A Badwan⁶

Affiliations

¹ The Jordanian Pharmaceutical Manufacturing Company (PLC), Suwagh Subsidiary for Drug Delivery Systems, P.O. Box 94, Naor 11710, Jordan. zkurdi@jpm.com.jo.
² Faculty of Engineering & Science, University of Greenwich, Medway Campus, Chatham Maritime, Kent ME44TB, UK. zkurdi@jpm.com.jo.
³ Faculty of Engineering & Science, University of Greenwich, Medway Campus, Chatham Maritime, Kent ME44TB, UK. b.z.chowdhry@greenwich.ac.uk.
⁴ Faculty of Engineering & Science, University of Greenwich, Medway Campus, Chatham Maritime, Kent ME44TB, UK. s.a.leharne@greenwich.ac.uk.
⁵ The Jordanian Pharmaceutical Manufacturing Company (PLC), Suwagh Subsidiary for Drug Delivery Systems, P.O. Box 94, Naor 11710, Jordan. momari@jpm.com.jo.
⁶ The Jordanian Pharmaceutical Manufacturing Company (PLC), Suwagh Subsidiary for Drug Delivery Systems, P.O. Box 94, Naor 11710, Jordan. jpm@go.com.jo.

Abstract

The aim of the work reported herein was to investigate the effect of various low molecular weight chitosans (LMWCs) on the stability of insulin using USP HPLC methods. Insulin was found to be stable in a polyelectrolyte complex (PEC) consisting of insulin and LMWC in the presence of a Tris-buffer at pH 6.5. In the presence of LMWC, the stability of insulin increased with decreasing molecular weight of LMWC; 13 kDa LMWC was the most efficient molecular weight for enhancing the physical and chemical stability of insulin. Solubilization of insulin-LMWC polyelectrolyte complex (I-LMWC PEC) in a reverse micelle (RM) system, administered to diabetic rats, results in an oral delivery system for insulin with acceptable bioactivity.

Publication types

Comparative Study

MeSH terms

Administration, Oral
Animals
Biological Availability
Biotransformation
Chitin / analogs & derivatives*
Chitin / chemistry
Chitosan
Diabetes Mellitus, Experimental / blood
Diabetes Mellitus, Experimental / drug therapy*
Diabetes Mellitus, Experimental / metabolism
Drug Delivery Systems*
Drug Liberation
Drug Stability
Drug Storage
Electrolytes / chemistry*
Excipients / chemistry*
Hyperglycemia / prevention & control
Hypoglycemic Agents / administration & dosage*
Hypoglycemic Agents / blood
Hypoglycemic Agents / pharmacokinetics
Hypoglycemic Agents / therapeutic use
Insulin / administration & dosage*
Insulin / analogs & derivatives
Insulin / analysis
Insulin / blood
Insulin / pharmacokinetics
Insulin / therapeutic use
Male
Micelles
Molecular Weight
Oligosaccharides
Particle Size
Random Allocation
Rats, Sprague-Dawley

Substances

Electrolytes
Excipients
Hypoglycemic Agents
Insulin
Micelles
Oligosaccharides
insulin, desamido (A21)-
oligochitosan
Chitin
Chitosan