Complete nucleotide sequence of phiCHU: a Luz24likevirus infecting Pseudomonas aeruginosa and displaying a unique host range

Damian J Magill; Olga V Shaburova; Elena N Chesnokova; Elena A Pleteneva; Victor N Krylov; Leonid A Kulakov

doi:10.1093/femsle/fnv045

Complete nucleotide sequence of phiCHU: a Luz24likevirus infecting Pseudomonas aeruginosa and displaying a unique host range

FEMS Microbiol Lett. 2015 May;362(9):fnv045. doi: 10.1093/femsle/fnv045. Epub 2015 Mar 30.

Authors

Damian J Magill¹, Olga V Shaburova², Elena N Chesnokova², Elena A Pleteneva², Victor N Krylov², Leonid A Kulakov³

Affiliations

¹ Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland, UK.
² Department of Microbiology, Laboratory for Genetics of Bacteriophages, I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow 105064, Russia.
³ Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland, UK l.kulakov@qub.ac.uk.

PMID: 25825475
DOI: 10.1093/femsle/fnv045

Abstract

A complete nucleotide sequence of the new Pseudomonas aeruginosa Luz24likevirus phiCHU was obtained. This virus was shown to have a unique host range whereby it grew poorly on the standard laboratory strain PAO1, but infected 26 of 46 clinical isolates screened, and strains harbouring IncP2 plasmid pMG53. It was demonstrated that phiCHU has single-strand interruptions in its genome. Analysis of the phiCHU genome also suggested that recombination event(s) participated in the evolution of the leftmost portion of the genome, presumably encoding early genes.

Keywords: antibiotic resistance; bacteriophage; phage therapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Genome, Viral*
Host Specificity*
Molecular Sequence Data
Open Reading Frames
Plasmids
Podoviridae / genetics*
Pseudomonas Phages / genetics*
Pseudomonas aeruginosa / virology*
Sequence Analysis, DNA

Associated data

GENBANK/KP233880