Abstract
The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.
Keywords:
calcium; cell biology; click chemistry; fluorescence; mouse; neuroscience; two-photon imaging.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Calcium / analysis*
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Calcium / chemistry
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Calcium Signaling
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Color
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Fluorescent Dyes / chemistry*
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Indicators and Reagents / chemical synthesis
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Indicators and Reagents / chemistry*
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Luminescent Measurements / methods
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Maleimides / chemistry
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Membrane Potentials
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Mice, Inbred C57BL
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Mice, Transgenic
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Microscopy, Fluorescence, Multiphoton / methods
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Models, Chemical
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Molecular Structure
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Neurons / chemistry*
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Neurons / metabolism
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Neurons / physiology
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Reproducibility of Results
Substances
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Alexa Fluor 488 C5-maleimide
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Bacterial Proteins
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Fluorescent Dyes
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Indicators and Reagents
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Luminescent Proteins
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Maleimides
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yellow fluorescent protein, Bacteria
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Calcium