3D surface topology guides stem cell adhesion and differentiation

Priyalakshmi Viswanathan; Matthew G Ondeck; Somyot Chirasatitsin; Kamolchanok Ngamkham; Gwendolen C Reilly; Adam J Engler; Giuseppe Battaglia

doi:10.1016/j.biomaterials.2015.01.034

3D surface topology guides stem cell adhesion and differentiation

Biomaterials. 2015 Jun:52:140-7. doi: 10.1016/j.biomaterials.2015.01.034. Epub 2015 Feb 24.

Authors

Priyalakshmi Viswanathan¹, Matthew G Ondeck², Somyot Chirasatitsin³, Kamolchanok Ngamkham⁴, Gwendolen C Reilly⁵, Adam J Engler⁶, Giuseppe Battaglia⁷

Affiliations

¹ Krebs Institute, The University of Sheffield, Sheffield S10 2TN, UK; Department of Biomedical Sciences, The University of Sheffield, Sheffield S10 2TN, UK.
² Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92037, USA; Material Science Program, University of California, San Diego, La Jolla, CA 92093, USA.
³ Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
⁴ Department of Chemistry, University College London, London WC1H 0AJ, UK; The MRC/UCL Centre for Medical Molecular Virology, University College London, London WC1H 0AJ, UK.
⁵ Department of Materials Science and Engineering, Insigneo Institute for in silico Medicine, The University of Sheffield, Sheffield S1 3JD, UK.
⁶ Sanford Consortium for Regenerative Medicine, University of California, San Diego, La Jolla, CA 92037, USA; Material Science Program, University of California, San Diego, La Jolla, CA 92093, USA; Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: aengler@ucsd.edu.
⁷ Department of Chemistry, University College London, London WC1H 0AJ, UK; The MRC/UCL Centre for Medical Molecular Virology, University College London, London WC1H 0AJ, UK. Electronic address: g.battaglia@ucl.ac.uk.

Abstract

Polymerized high internal phase emulsion (polyHIPE) foams are extremely versatile materials for investigating cell-substrate interactions in vitro. Foam morphologies can be controlled by polymerization conditions to result in either open or closed pore structures with different levels of connectivity, consequently enabling the comparison between 2D and 3D matrices using the same substrate with identical surface chemistry conditions. Additionally, here we achieve the control of pore surface topology (i.e. how different ligands are clustered together) using amphiphilic block copolymers as emulsion stabilizers. We demonstrate that adhesion of human mesenchymal progenitor (hES-MP) cells cultured on polyHIPE foams is dependent on foam surface topology and chemistry but is independent of porosity and interconnectivity. We also demonstrate that the interconnectivity, architecture and surface topology of the foams has an effect on the osteogenic differentiation potential of hES-MP cells. Together these data demonstrate that the adhesive heterogeneity of a 3D scaffold could regulate not only mesenchymal stem cell attachment but also cell behavior in the absence of soluble growth factors.

Keywords: Cell signaling; Osteogenesis; Stem cell; Surface topology.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biocompatible Materials / chemistry*
Cell Adhesion
Cell Differentiation
Cell Line
Cell Proliferation
Humans
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism
Osteogenesis
Polymers / chemistry
Porosity
Tissue Scaffolds / chemistry*

Substances

Biocompatible Materials
Polymers

Abstract

Publication types

MeSH terms

Substances

Grants and funding