Molecular cloning of the BLADE-ON-PETIOLE gene and expression analyses during nodule development in Lupinus luteus

Kamil Frankowski; Emilia Wilmowicz; Agata Kućko; Agnieszka Zienkiewicz; Krzysztof Zienkiewicz; Jan Kopcewicz

doi:10.1016/j.jplph.2015.01.019

Molecular cloning of the BLADE-ON-PETIOLE gene and expression analyses during nodule development in Lupinus luteus

J Plant Physiol. 2015 May 1:179:35-9. doi: 10.1016/j.jplph.2015.01.019. Epub 2015 Mar 12.

Authors

Kamil Frankowski¹, Emilia Wilmowicz², Agata Kućko³, Agnieszka Zienkiewicz⁴, Krzysztof Zienkiewicz⁵, Jan Kopcewicz⁶

Affiliations

¹ Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland. Electronic address: kfrank@o2.pl.
² Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 4 Wileńska Street, 87-100 Toruń, Poland. Electronic address: emwil@umk.pl.
³ Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland; Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 4 Wileńska Street, 87-100 Toruń, Poland. Electronic address: kuckoa@poczta.onet.pl.
⁴ Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland; Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008, Granada, Spain. Electronic address: agazet@umk.pl.
⁵ Department of Cell Biology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland; Department of Biochemistry, Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, 18008, Granada, Spain. Electronic address: kzienk@umk.pl.
⁶ Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 1 Lwowska Street, 87-100 Toruń, Poland. Electronic address: kopcew@umk.pl.

PMID: 25817415
DOI: 10.1016/j.jplph.2015.01.019

Abstract

The BLADE-ON-PETIOLE (BOP) genes have been recently shown to play an essential role in many physiological processes, including embryogenesis, meristem determinacy, leaf patterning and nodule development. In our research we used Lupinus luteus, a plant with great agronomic potential due to its high protein content and nitrogen fixation ability. In this work, LlBOP in L. luteus was identified for the first time and its expression during nodule development was analyzed. The high expression levels of LlBOP and LlLbI (LEGHEMOGLOBIN), essential to nitrogen-fixing symbiosis, were noted in the developing root nodules and were correlated with the occurrence of leghemoglobin. All of these data indicate that LlBOP is an important regulator of root nodule formation and functioning in L. luteus.

Keywords: BLADE-ON-PETIOLE; Leghemoglobin; Lupinus luteus; Nodules.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary / genetics
DNA, Complementary / isolation & purification
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant*
Genes, Plant*
Lupinus / genetics*
Lupinus / growth & development*
Molecular Sequence Data
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / metabolism
Plant Root Nodulation / genetics
Root Nodules, Plant / genetics*
Root Nodules, Plant / growth & development*

Substances

DNA, Complementary
Plant Proteins