Structure elucidation of the capsular polysaccharide of Acinetobacter baumannii AB5075 having the KL25 capsule biosynthesis locus

Carbohydr Res. 2015 May 18:408:8-11. doi: 10.1016/j.carres.2015.02.011. Epub 2015 Mar 5.

Abstract

Capsular polysaccharide was isolated by the phenol-water extraction of Acinetobacter baumannii AB5075 and studied by 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear trisaccharide repeating unit was established: → 3)-β-D-ManpNAcA-(1 → 4)-β-D-ManpNAcA-(1 → 3)-α-D-QuipNAc4NR-(1 → where R indicates (S)-3-hydroxybutanoyl or acetyl in the ratio ∼ 2.5:1. The genes in the polysaccharide biosynthesis locus designated KL25 are appropriate to the established CPS structure.

Keywords: Acinetobacter baumannii; Capsular polysaccharide structure; Polysaccharide gene locus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter baumannii / chemistry
  • Acinetobacter baumannii / genetics*
  • Acinetobacter baumannii / metabolism
  • Bacterial Capsules / chemistry
  • Bacterial Capsules / genetics
  • Bacterial Capsules / metabolism*
  • Carbohydrate Sequence
  • Genes, Bacterial
  • Genetic Loci
  • Magnetic Resonance Spectroscopy
  • Polysaccharides, Bacterial / chemistry*
  • Polysaccharides, Bacterial / genetics

Substances

  • Polysaccharides, Bacterial