Supercritical synthesis of poly (2-dimethylaminoethyl methacrylate)/ferrite nanocomposites for real-time monitoring of protein release

Abstract

A supercritical carbon dioxide (SCC)-assisted process was developed to synthesize protein-supported poly (2-dimethylaminoethyl methacrylate)/ferrite nanocomposites (PNCs). The process involve 2,2-azobisisobutyronitrile-initiated in situ polymerization of 2-dimethylaminoethyl methacrylate in presence of ferrite nanoparticles and bisacrylamide at 90 ± 1 °C, 1200 psi over 6 h in SCC. This was followed by subsequent loading of bovine serum albumin (BSA) as a model protein over PNCs in phosphate buffer (PBS, pH 7.4) at 1200 psi, 35 ± 1 °C over additional 2 h in SCC. The formation of PNCs was ascertained through ultraviolet-visible, Fourier transform-infrared, X-ray diffraction spectra, transmission electron, atomic force microscopy and magnetometry. The developed process extends large scale production of nanomagnetic PNCs suitable as carrier for protein release applications with optimal release properties. The release of protein from PNCs under in vitro in PBS down to nanomolar range with high temporal resolution, speed and reproducibility was quantified through square wave voltammetry.