Functional elucidation of miR-494 in the tumorigenesis of nasopharyngeal carcinoma

Tumour Biol. 2015 Sep;36(9):6679-89. doi: 10.1007/s13277-015-3356-8. Epub 2015 Mar 26.

Abstract

Nasopharyngeal carcinoma has very high incidence and high mortality worldwide. MiRNA is related to the tumorigenesis and metastasis of a variety of tumors. In the present study, we verify that the expression of miR-494 in NPC tissues and NPC-derived cells was down-regulated, respectively. The proliferation, colony formation, migration, and invasion of NPC-derived cells were suppressed, while the cell apoptosis was promoted, when miR-494 was over-expressed in these cells. GALNT7 and CDK16 were confirmed to be the direct targets of miR-494. These results suggested that miR-494 play an inhibitory role in the tumorigenesis of NPC.

Keywords: CDK16; GALNT7; Nasopharyngeal carcinoma; Tumor suppressor; miR-494.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics
  • Carcinogenesis / genetics
  • Carcinoma
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Cyclin-Dependent Kinases / genetics*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs / biosynthesis*
  • MicroRNAs / genetics
  • N-Acetylgalactosaminyltransferases / genetics*
  • Nasopharyngeal Carcinoma
  • Nasopharyngeal Neoplasms / genetics*
  • Nasopharyngeal Neoplasms / pathology
  • Neoplasm Invasiveness / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics

Substances

  • MIRN494 microRNA, human
  • MicroRNAs
  • RNA, Messenger
  • N-Acetylgalactosaminyltransferases
  • UDP-N-acetylgalactosamine--polypeptide N-acetylgalactosaminyltransferase 7
  • Cyclin-Dependent Kinases
  • PCTAIRE-1 protein kinase