A glycol methacrylate method for the routine histologic evaluation of rat inner ear

Stain Technol. 1985 Mar;60(2):63-8. doi: 10.3109/10520298509113894.

Abstract

A method has been developed for the histologic evaluation of rat inner ear using glycol methacrylate (GMA) and steel knife sectioning. The necropsy, fixation, and histologic techniques described are not so complex and difficult as to preclude their routine use. The method is particularly useful in studies in which the entire inner ear of a large number of animals must be evaluated histologically.

MeSH terms

  • Animals
  • Dissection / methods
  • Ear, Inner / anatomy & histology*
  • Ear, Inner / cytology
  • Evaluation Studies as Topic
  • Hair Cells, Auditory / cytology
  • Histological Techniques*
  • Microtomy / methods
  • Polyhydroxyethyl Methacrylate / pharmacology*
  • Polymethacrylic Acids / pharmacology*
  • Rats
  • Staining and Labeling / methods

Substances

  • Polymethacrylic Acids
  • Polyhydroxyethyl Methacrylate