CARB-17 family of β-lactamases mediates intrinsic resistance to penicillins in Vibrio parahaemolyticus

Jiachi Chiou; Ruichao Li; Sheng Chen

doi:10.1128/AAC.00047-15

CARB-17 family of β-lactamases mediates intrinsic resistance to penicillins in Vibrio parahaemolyticus

Antimicrob Agents Chemother. 2015;59(6):3593-5. doi: 10.1128/AAC.00047-15. Epub 2015 Mar 23.

Authors

Jiachi Chiou¹, Ruichao Li¹, Sheng Chen²

Affiliations

¹ Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, People's Republic of China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
² Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, People's Republic of China State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong sheng.chen@polyu.edu.hk.

Abstract

Vibrio parahaemolyticus is commonly resistant to ampicillin, yet the mechanisms underlying this phenomenon are not clear. In this study, a novel class A carbenicillin-hydrolyzing β-lactamase (CARB) family of β-lactamases, bla(CARB-17), was identified and found to be responsible for the intrinsic penicillin resistance in V. parahaemolyticus. Importantly, bla(CARB-17)-like genes were present in all 293 V. parahaemolyticus genome sequences available in GenBank and detectable in all 91 V. parahaemolyticus food isolates, further confirming the intrinsic nature of this gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbenicillin / pharmacology
Microbial Sensitivity Tests
Penicillin Resistance / genetics
Penicillins / pharmacology*
Phylogeny
Vibrio parahaemolyticus / drug effects*
Vibrio parahaemolyticus / enzymology*
beta-Lactamases / classification
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Penicillins
beta-Lactamases
Carbenicillin