Oxidation of plastohydroquinone by photosystem II and by dioxygen in leaves

Abstract

In sunflower leaves linear electron flow LEF=4O2 evolution rate was measured at 20 ppm O2 in N2. PSII charge separation rate CSRII=aII∙PAD∙(Fm-F)/Fm, where aII is excitation partitioning to PSII, PAD is photon absorption density, Fm and F are maximum and actual fluorescence yields. Under 630 nm LED+720 nm far-red light (FRL), LEF was equal to CSRII with aII=0.51 to 0.58. After FRL was turned off, plastoquinol (PQH2) accumulated, but LEF decreased more than accountable by F increase, indicating PQH2-oxidizing cyclic electron flow in PSII (CEFII). CEFII was faster under conditions requiring more ATP, consistent with CEFII being coupled with proton translocation. We propose that PQH2 bound to the QC site is oxidized, one e- moving to P680+, the other e- to Cyt b559. From Cyt b559 the e- reduces QB- at the QB site, forming PQH2. About 10-15% electrons may cycle, causing misses in the period-4 flash O2 evolution and lower quantum yield of photosynthesis under stress. We also measured concentration dependence of PQH2 oxidation by dioxygen, as indicated by post-illumination decrease of Chl fluorescence yield. After light was turned off, F rapidly decreased from Fm to 0.2 Fv, but further decrease to F0 was slow and O2 concentration dependent. The rate constant of PQH2 oxidation, determined from this slow phase, was 0.054 s(-1) at 270 μM (21%) O2, decreasing with Km(O2) of 60 μM (4.6%) O2. This eliminates the interference of O2 in the measurements of CEFII.

Keywords: Cyclic electron transport; Leaves; Photosystem II.