The initial inflammatory response to bioactive implants is characterized by NETosis

PLoS One. 2015 Mar 23;10(3):e0121359. doi: 10.1371/journal.pone.0121359. eCollection 2015.

Abstract

Implants trigger an inflammatory response, which is important for osseointegration. Here we studied neutrophil extracellular trap (NET) release of human neutrophils in response to sandblasted large-grit acid etched (SLA) implants using fluorescent, confocal laser scanning and scanning electron microscopy. Our studies demonstrate that human neutrophils rapidly adhered to SLA surfaces, which triggered histone citrullination and NET release. Further studies showed that albumin or acetylsalicylic acid had no significant effects on the inflammatory response to SLA surfaces. In contrast to bioinert materials, which do not osseointegrate, the bioactivity of SLA surfaces is coupled with the ability to release NETs. Further investigations are necessary for clarifying the role of NETosis for osseointegration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Extracellular Traps / drug effects*
  • Female
  • Histones / metabolism
  • Humans
  • Implants, Experimental / adverse effects*
  • Inflammation / etiology
  • Male
  • Neutrophils / drug effects*
  • Neutrophils / metabolism
  • Neutrophils / ultrastructure
  • Osseointegration
  • Titanium / pharmacology*

Substances

  • Histones
  • Titanium

Grants and funding

The authors acknowledge support by Deutsche Forschungsgemeinschaft and Open Access Publishing Fund of Tuebingen University. The authors thank the Dental Ratio Company (Langenfeld, NRW, Germany) for supply of SLA titanium test plates and financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.