Optical-resolution photoacoustic endomicroscopy in vivo

Joon-Mo Yang; Chiye Li; Ruimin Chen; Bin Rao; Junjie Yao; Cheng-Hung Yeh; Amos Danielli; Konstantin Maslov; Qifa Zhou; K Kirk Shung; Lihong V Wang

doi:10.1364/BOE.6.000918

Optical-resolution photoacoustic endomicroscopy in vivo

Biomed Opt Express. 2015 Feb 23;6(3):918-32. doi: 10.1364/BOE.6.000918. eCollection 2015 Mar 1.

Authors

Affiliations

¹ Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130, USA ; These authors contributed equally to this work.
² NIH Ultrasonic Transducer Resource Center, Department of Biomedical Engineering, University of Southern California, 1042 Downey Way, University Park, Los Angeles, California 90089, USA ; These authors contributed equally to this work.
³ Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130, USA.
⁴ Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130, USA ; Currently with the Faculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, Israel.
⁵ NIH Ultrasonic Transducer Resource Center, Department of Biomedical Engineering, University of Southern California, 1042 Downey Way, University Park, Los Angeles, California 90089, USA.

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) has become a major experimental tool of photoacoustic tomography, with unique imaging capabilities for various biological applications. However, conventional imaging systems are all table-top embodiments, which preclude their use in internal organs. In this study, by applying the OR-PAM concept to our recently developed endoscopic technique, called photoacoustic endoscopy (PAE), we created an optical-resolution photoacoustic endomicroscopy (OR-PAEM) system, which enables internal organ imaging with a much finer resolution than conventional acoustic-resolution PAE systems. OR-PAEM has potential preclinical and clinical applications using either endogenous or exogenous contrast agents.

Keywords: (170.0170) Medical optics and biotechnology; (170.0180) Microscopy; (170.2150) Endoscopic imaging; (170.3880) Medical and biological imaging; (170.3890) Medical optics instrumentation; (170.5120) Photoacoustic imaging.

Abstract

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