Nature-inspired enzymatic cascades to build valuable compounds

Renata Sigrist; Bruna Zucoloto da Costa; Anita Jocelyne Marsaioli; Luciana Gonzaga de Oliveira

doi:10.1016/j.biotechadv.2015.03.010

Nature-inspired enzymatic cascades to build valuable compounds

Biotechnol Adv. 2015 Sep-Oct;33(5):394-411. doi: 10.1016/j.biotechadv.2015.03.010. Epub 2015 Mar 18.

Authors

Renata Sigrist¹, Bruna Zucoloto da Costa¹, Anita Jocelyne Marsaioli¹, Luciana Gonzaga de Oliveira²

Affiliations

¹ Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil.
² Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil. Electronic address: luciana@iqm.unicamp.br.

PMID: 25795056
DOI: 10.1016/j.biotechadv.2015.03.010

Abstract

Biocatalysis currently is focusing on enzymatic and multi-enzymatic cascade processes instead of single steps imbedded into chemical pathways. Alongside this scientific revolution, this review provides an overview on multi-enzymatic cascades that are responsible for the biosynthesis of some terpenes, alkaloids and polyethers, which are important classes of natural products. Herein, we illustrate the development of studies inspired by multi- and chemo-enzymatic approaches to build the core moieties of polyethers, polypeptide alkaloids, piperidines and pyrrolidines promoted by the joint action of oxidoreductases, hydrolases, cyclases, transaminases and imine reductases.

Keywords: Chemo- and multi-enzymatic processes; Cyclases; Hydrolases; Imine reductases; Oxidoreductases; Piperidines and pyrrolidines; Polyethers; Polypeptide alkaloids; Transaminases.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Alkaloids / metabolism
Biocatalysis
Biosynthetic Pathways*
Biotechnology*
Enzymes*

Substances

Alkaloids
Enzymes