Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS

Anna Vallverdú-Queralt; Nuria Boix; Ester Piqué; Jesús Gómez-Catalan; Alexander Medina-Remon; Gemma Sasot; Mercè Mercader-Martí; Juan M Llobet; Rosa M Lamuela-Raventos

doi:10.1016/j.foodchem.2015.02.098

Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS

Food Chem. 2015 Aug 15:181:146-51. doi: 10.1016/j.foodchem.2015.02.098. Epub 2015 Feb 25.

Authors

Anna Vallverdú-Queralt¹, Nuria Boix², Ester Piqué³, Jesús Gómez-Catalan⁴, Alexander Medina-Remon⁵, Gemma Sasot⁶, Mercè Mercader-Martí⁷, Juan M Llobet⁸, Rosa M Lamuela-Raventos⁹

Affiliations

¹ INRA, UMR1083 Sciences pour l'œnologie, 2 Place Pierre Viala, Montpellier Cedex 34060, France; Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III, Sinesio Delgado 4, 28029 Madrid, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: avallverdu@ub.edu.
² GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: nuriaboix@ub.edu.
³ GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: esterpique@ub.edu.
⁴ GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: jesusgomez@ub.edu.
⁵ Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III, Sinesio Delgado 4, 28029 Madrid, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain; Nutrition and Food Science Department, XaRTA, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: amedina@ub.edu.
⁶ Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III, Sinesio Delgado 4, 28029 Madrid, Spain; Nutrition and Food Science Department, XaRTA, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: gemmamsf@gmail.com.
⁷ Miguel Torres, Comercio 22, 08720 Vilafranca del Penedes, Barcelona, Spain. Electronic address: mmercader@torres.es.
⁸ GRET-CERETOX and Toxicology Unit, Pharmacology and Therapeutical Chemistry, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain. Electronic address: jmllobet@ub.edu.
⁹ Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición (ciberobn), Instituto de Salud Carlos III, Sinesio Delgado 4, 28029 Madrid, Spain; INSA-UB, Nutrition and Food Safety Research Institute, University of Barcelona, Food and Nutrition Torribera Campus, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain; Nutrition and Food Science Department, XaRTA, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, Spain. Electronic address: lamuela@ub.edu.

PMID: 25794733
DOI: 10.1016/j.foodchem.2015.02.098

Abstract

The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry.

Keywords: Identification; Orbitrap-MS; Polyphenols; Red wine extract; Zebrafish (ZF).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatography, High Pressure Liquid / methods*
Chromatography, Liquid / methods*
Mass Spectrometry / methods*
Phenols / analysis*
Polyphenols / chemistry*
Spectrometry, Mass, Electrospray Ionization / methods
Wine / analysis*
Zebrafish / physiology*

Substances

Phenols
Polyphenols