Gene expression knockdown by transfection of siRNAs into mammalian cells

Yoann Abel; Mathieu Rederstorff

doi:10.1007/978-1-4939-2547-6_18

Gene expression knockdown by transfection of siRNAs into mammalian cells

Methods Mol Biol. 2015:1296:199-202. doi: 10.1007/978-1-4939-2547-6_18.

Authors

Yoann Abel¹, Mathieu Rederstorff

Affiliation

¹ CNRS UMR 7365 IMoPA, Biopôle, Université de Lorraine, 9 avenue de la Forêt de Haye, CS 50184, Vandoeuvre-lès-Nancy, 54506, France.

PMID: 25791602
DOI: 10.1007/978-1-4939-2547-6_18

Abstract

SiRNA induced gene silencing or RNA interference is a powerful tool to knock down gene expression and perform gene function studies. In this chapter, we describe a basic method to silence gene expression by transfecting a specific synthetic siRNA into mammalian HeLa cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

HeLa Cells
Humans
RNA Interference*
RNA, Small Interfering / genetics*
Synthetic Biology / methods
Transfection / methods*

Substances

RNA, Small Interfering