Direct cloning of double-stranded RNAs

Methods Mol Biol. 2015:1296:53-64. doi: 10.1007/978-1-4939-2547-6_6.

Abstract

Most annotated genomes show a large number of sense-antisense transcripts that can generate double-stranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Base Pairing
  • Base Sequence
  • Cloning, Molecular / methods*
  • High-Throughput Nucleotide Sequencing
  • Molecular Sequence Data
  • Molecular Structure
  • RNA, Double-Stranded / chemistry
  • RNA, Double-Stranded / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription, Genetic / physiology

Substances

  • RNA, Double-Stranded